Influence of adding Protein Hydrolysate on Gelatin Film Characteristics and Its Anti-Browning Efficacy on Fruit Surfaces
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Abstract
The objective of this study was to investigate the impact of protein hydrolysate on the characteristics of gelatin films. The enzymatically hydrolyzing soybean meal with bromelain was used to produce the protein hydrolysate. A comparative investigation was carried out to assess both the physical properties and anti-browning reactions of gelatin films depending on the different concentrations of protein hydrolysate at 0.1%, 0.5%, 1%, and 3%. The resul ts demonstrated that all aspects of the film quality tended to increase depending on the amount of protein hydrolysate added. Films mixed with 3% protein hydrolysate have tensile strength and water vapor permeability with values of 39.50 MPa and 1.15 g mm/kPa h−1 m−2, respectively. The film containing protein hydrolysate became thicker and darker yellow with an increasing amount of protein hydrolysate. The experiment into the anti-browning reaction on sliced apple skin revealed that films containing protein hydrolysate were able to delay the browning reaction more than those lacking this component. The inhibition's efficacy increased proportionally with increasing protein hydrolysate concentrations. These findings were obtained under regulated storage settings of 4 °C. As a result, the gelatin film combined with protein hydrolysate has the ability to delay the browning reaction.
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References
สำนักงานมาตรฐานผลิตภัณฑ์อุตสาหกรรม. (2562, 27 ธันวาคม). พระราชบัญญัติมาตรฐานผลิตภัณฑ์อุตสาหกรรม พ.ศ. 2511. https://www.tisi.go.th/data/law/pdf_files/law1/tip15july19_2.pdf
Aitboulahsen, M., Zantar, S., Laglaoui, A., Chairi, H., Arakrak, A., Bakkali, M., & Hassani Zerrouk, M. (2018). Gelatin-based edible coating combined with Mentha pulegium essential oil as bioactive packaging for strawberries. Journal of food quality, 2018, Article ID 8408915, 1-7. https://doi.org/10.1155/2018/8408915
Amjadi, S., Emaminia, S., Heyat Davudian, S., Pourmohammad, S., Hamishehkar, H., & Roufegarinejad, L. (2019). Preparation and characterization of gelatin-based nanocomposite containing chitosan nanofiber and ZnO nanoparticles. Carbohydrate polymers, 216, 376-384.
Asquith, R. S., & Brooke, K. E. (1966). The formation of dihydroxyphenylalanine on exposure of tyrosine to ultraviolet radiation. Journal of the society of dyers and colourists, 82, 226.
Aydin, S., Gunduz, A., & Sahin, F. (2017). Lipid-based edible films. The journal of scientific and engineering research, 4, 86-92.
Cai, L., Shi, H., Cao, A., & Jia, J. (2019). Characterization of gelatin/chitosan polymer films integrated with docosahexaenoic acids fabricated by different methods. Scientific reports, 9(1), 8375. https://doi.org/10.1038/s41598-019-44807-x
Freeberg, S. (2019). Edible films: A review of recent research. Food science &nutrition, 7(12), 4397-4412.
Hamdan, N., Lee, C. H., Wong, S. L., Fauzi, C. E. N. C. A., Zamri, N. M. A., & Lee, T. H. (2022). Prevention of enzymatic browning by natural extracts and genome-editing: A review on recent progress. Molecules, 27(3), 1101. https://doi.org/10.3390/molecules27031101
Ji, Y., Wang, Z., Deng, Q., Chen, J., He, Z., Zeng, M., Qin, F., & Pan, H. (2023). Soy protein hydrolysates affect the structural and mechanical properties of soy protein-wheat gluten extrudates using high moisture extrusion. Foods, 12(5), 912. https://doi.org/10.3390/foods12050912
Kchaou, H., Jridi, M., Benbettaieb, N., Debeaufort, F., & Nasri, M. (2020). Bioactive films based on cuttlefish (Sepia officinalis) skin gelatin incorporated with cuttlefish protein hydrolysates: Physicochemical characterization and antioxidant properties. Food packaging and shelf life, 24, 100477. https://doi.org/ 10.1016/j.fpsl.2020.100477
Khan, S., Khan, A., & Khan, R. (2019). Edible films and coatings: A review. Food science & technology, 97, 253-266.
Knoll, J., & Steinbüchel, A. (2010). Edible films and coatings: Materials, applications, and perspectives. Trends in food science & technology, 21(1), 40-52.
Laohakunjit, N. & Noomhorm, A. (2004). Effect of plasticizer on mechanical and barrier properties of rice starch film. Starch-Starke, 56, 348-356.
Lee, J. H., Park, D. Y., & Kim, H. S. (2020). Alginate-based edible films for foodpackaging: A comprehensive review. Journal of food hydrocolloids, 45, 122-130.
Liu, X., Lu, Y., Yang, Q., Yang, H., Li, Y., Zhou, B., Li, T., Gao, Y., & Qiao, L. (2018). Cod peptides inhibit browning in fresh-cut potato slices: A potential anti-browning agent of random peptides for regulating food properties. Postharvest biology and technology, 146, 36-42. https://doi.org/10.1016/j.postharvbio.2018.08.001
Maryam Adilah, Z. A., & Nur Hanani, Z. A. (2016). Active packaging of fish gelatin films with Morinda citrifolia oil. Food bioscience, 16, 66-71. https://doi.org/https://doi.org/10.1016/j.fbio.2016.10.002
Norizah Mhd Sarbon. (2011). Nutritional and physicochemical properties of chicken proteins and peptides. [Doctoral dissertation, University of Surrey]. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589971
Sarbon, Badii, F., & Howell, N. (2013). Preparation and characterization of chicken skin gelatin as an alternative to mammalian gelatin. Food hydrocolloids, 30(1), 143–151. https://doi.org/10.1016/j.foodhyd.2012.05.009
Singh, R., & Singh, N. (2019). Edible films and coatings: A review of recent developments. Trends in food science & technology, 89, 39-53.
Wang, L., Ding, J., Fang, Y., Pan, X., Fan, F., Li, P., & Hu, Q. (2020). Effect of ultrasonic power on properties of edible composite films based on rice protein hydrolysates and chitosan. Ultrasonics sonochemistry, 65, 105049. https://doi.org/https://doi.org/10.1016/j.ultsonch.2020.105049
Yang, S., Li, H., & Sun, H. (2018). Preparation of gelatin-based films modified with nanocrystalline cellulose. Iranian polymer journal, 27(9), 645-652. https://doi.org/10.1007/s13726-018-0641-6