THE BIOCOMPATIBILITY OF BLUE LIGHT ACTIVATED METHACRYLATED HYALURONIC ACID HYDROGEL WITH MC3T3-E1 MOUSE OSTEOBLASTIC CELL LINE

Abstract

Bone plays a crucial role in the success of dental implant placement. While the use of bone grafting materials has encountered certain limitations and biomaterial as tissue engineering scaffold is one of interest. This study aimed to evaluate the biocompatibility of MC3T3-E1 cell line and blue light activated hyaluronic acid (HA) hydrogel which was designed to be used as scaffold. The hydrogel was fabricated from methacrylated hyaluronic acid (MeHA) cross-linked by LAP and activated by blue light. The biological properties of hydrogels were performed in vitro model, consisted of proliferation in three-dimensionally encapsulated cell culture. The results revealed that blue light activated HA hydrogel was found to be biocompatible with mouse osteoblastic cell line (MC3T3-E1 cell). Therefore, the utilization of a blue light activated HA hydrogel both as a scaffold and drug delivery system for bone tissue engineering could be a promising approach. In addition, this could be translated into in vivo model for further investigation.