OSTEOGENSIS MIMICKING SCAFFOLDS FOR BOME TISSUE ENGINEERING

Abstract

Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degeneration and inflammation, which can affect various joints throughout the body. In severe cases, surgical intervention may be required, with tissue engineering scaffolds offering a potential approach for tissue replacement and regeneration. In this study, a biphasic scaffold consisting of a silk fibroin film affixed to a silk fibroin/magnesium chloride (MgCl₂) sponge was developed for potential application in osteoarthritis treatment. Previous studies have investigated silk fibroin film combined with collagen type I and polyvinyl alcohol (PVA). Therefore, this study aimed to mimic the functional extracellular matrix (ECM) using a silk fibroin film and a collagen type I/PVA/MgCl₂ scaffold to support bone tissue regeneration. The scaffold morphology was characterized using scanning electron microscopy (SEM). The biological functionality of the scaffolds was evaluated using bone cell culture, and cell proliferation was assessed to determine cellular responses to the scaffolds. In addition, Fourier-transform infrared (FT-IR) spectroscopy was used to characterize the chemical functional groups and molecular interactions within the scaffolds. The silk fibroin film and collagen type I/PVA/MgCl₂ scaffold demonstrated potential for improving scaffold performance, particularly in terms of cell adhesion and proliferation. These findings suggest that the developed scaffold has potential as a tissue engineering scaffold for bone tissue regeneration and may be applicable to the treatment of osteoarthritis.